Practical two: Buffer Solutions Drug Discovery & Development BHS005-6 Theory: Refer to lecture notes. “Physicochemical properties of Drugs”. The following equations will also be required: pH = pKa + log([base]/[acid]); pKa = -log(Ka); Method Using pipettes, accurately prepare the following solutions: 30ml 0.1M CH3COONa + 30ml 0.1M CH3COOH20ml 0.1M CH3COONa + 50ml 0.1M CH3COOH10ml 0.1M CH3COONa + 50ml 0.1M CH3COOH50ml 0.1M CH3COONa + 100ml 0.1M CH3COOH Using a calibrated pH meter, measure the pH of the three solutions A, B and C. Use this data to calculate the pKa and Ka values for ethanoic acid. Note the temperature at which the values were obtained and compare these to the literature values.Titrate 50ml of solution D with 0.1M HCl, adding the acid in 2ml aliquots and recording the pH after each addition. Add a total of 40ml of 0.1M HCl. 50ml of Solution DpH value+2ml of 0.1M HCl (total 52 ml)3.99+2ml of 0.1M HCl (total 54 ml)3.67+2ml of 0.1M HCl (total 56 ml)3.59+2ml of 0.1M HCl (total 58 ml)3.22+2ml of 0.1M HCl (total 60 ml)2.66+2ml of 0.1M HCl (total 62 ml)2.22+2ml of 0.1M HCl (total 64 ml)1.97+2ml of 0.1M HCl (total 66 ml)1.65+2ml of 0.1M HCl (total 68 ml)1.59+2ml of 0.1M HCl (total 70 ml)1.58+2ml of 0.1M HCl (total 72 ml)1.57+2ml of 0.1M HCl (total 74 ml)1.5+2ml of 0.1M HCl (total 76 ml)1.47+2ml of 0.1M HCl (total 78 ml)1.39+2ml of 0.1M HCl (total 80 ml)1.35+2ml of 0.1M HCl (total 82 ml)1.25+2ml of 0.1M HCl (total 84 ml)1.24+2ml of 0.1M HCl (total 86 ml)1.18+2ml of 0.1M HCl (total 88 ml)1.17+2ml of 0.1M HCl (total 90 ml)1.16 Titrate 50ml of solution D with 0.1M NaOH, adding the alkali in 2ml aliquots and recording the pH after each addition. Add a total of 40ml of 0.1M NaOH. 50ml of Solution DpH value+2ml of 0.1M NaOH (total 52 ml)4.26+2ml of 0.1M NaOH (total 54 ml)4.28+2ml of 0.1M NaOH (total 56 ml)4.36+2ml of 0.1M NaOH (total 58 ml)4.43+2ml of 0.1M NaOH (total 60 ml)4.49+2ml of 0.1M NaOH (total 62 ml)4.58+2ml of 0.1M NaOH (total 64 ml)4.63+2ml of 0.1M NaOH (total 66 ml)4.68+2ml of 0.1M NaOH (total 68 ml)4.76+2ml of 0.1M NaOH (total 70 ml)4.95+2ml of 0.1M NaOH (total 72 ml)5.00+2ml of 0.1M NaOH (total 74 ml)5.09+2ml of 0.1M NaOH (total 76 ml)5.25+2ml of 0.1M NaOH (total 78 ml)5.39+2ml of 0.1M NaOH (total 80 ml)5.62+2ml of 0.1M NaOH (total 82 ml)5.92+2ml of 0.1M NaOH (total 84 ml)6.75+2ml of 0.1M NaOH (total 86 ml)11.03+2ml of 0.1M NaOH (total 88 ml)11.4+2ml of 0.1M NaOH (total 90 ml)11.59 Plot graphs of pH against volume of 0.1M HCl and pH against volume of 0.1M NaOH on the same sheet of graph paper and calculate the buffer capacity with respect to both acid and alkali.

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